A modular approach, using biocompatible synthetic materials to mimic viral vectors for efficient DNA delivery, may be useful. The modular approach requires at least two components in a DNA delivery system; each component functions so that barriers to DNA delivery, such as low uptake of DNA molecules by cells or lack of nuclear targeting, can be ...

A modular and hierarchical DNA assembly platform for synthetic biology based on Golden Gate (Type IIS restriction enzyme) cloning is described. This enabling technology, termed MIDAS (for Modular ...

Bacillus subtilis is a valuable industrial production platform for proteins, a bacterial model for cellular differentiation and its endospores have been proposed as a vehicle for vaccine delivery. As such B. subtilis is a major synthetic biology chassis but, unlike Escherichia coli, lacks a standardized toolbox for genetic manipulation. EcoFlex is a versatile modular DNA assembly toolkit for ...

Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes

Abstract. Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method.The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1].

Here we demonstrate the automated modular assembly of 96 DNA devices from various basic DNA parts (Figure 1b) using two automated robotic liquid handling platforms. Each transcriptional unit is a linear arrangement of promoter, ribosomal binding site, gene, and transcriptional terminator, cloned into a specific destination vector.

Deoxyribonucleic acid (DNA), a genetic material, encodes all living information and living characteristics, e.g., in cell, DNA signaling circuits control the transcription activities of specific genes. In recent years, various DNA circuits have been developed to implement a wide range of signaling and for regulating gene network functions. In particular, a synthetic DNA circuit, with a ...

Modular self-assembly of gamma-modified peptide nucleic acids in organic solvent mixtures ... Maier, A. M. et al. Self-assembled DNA tubes …

CITIIC REPORTS 61636 I 1.13srep1636 1 A Modular Assembly Platform for Rapid Generation of DNA Constructs Elliot H. Akama-Garren1,2,*, NikhilS.Joshi1,*, Tuomas Tammela1, GregoryP.Chang1, Bethany L. Wagner1, Da-YaeLee 1, William M.Rideout III 1, Thales Papagiannakopoulos1, Wen Xue3 & Tyler Jacks1,2,4 Traditional cloning …

In this approach, DNA probes are labeled with poorly fluorescent precursors and assembled with target nucleic acids into catalytic hybrids. These then chemically convert the precursors into fluorescent reporters[ 9 ] (e.g. through the Staudinger reaction,[ 10 ] transthioesterification,[ 11 ] and aminolysis).[ 12 ]

Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method. The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1]. T …

DNA Programmed Assembly of Cells (DPAC) is a powerful tissue engineering technique that patterns cells at single-cell resolution with precise control over cell-cell spacing and tissue geometry. In DPAC, cell membranes are decorated with DNA oligonucleotides (oligos) using two lipid-modified oligos designed to hybridize on the cell membrane.

Abstract. Previous work has shown the ability of the Echo Liquid Handler to generate DNA constructs using various assembly chemistries with a miniaturized protocol 1,2.Interchanging smaller, modular pieces of DNA is the preferred method for numerous workflows due to decreased synthesis cost and faster progression along the design, build, test, and learn cycle.

2 TECHNICAL NOTE Modular DNA Assembly of PIK3CA Using Acoustic Liquid Handling Linear Vector DNA inserts with 15–30 bp overlapping ends (PCR-amplified) Incubate at 50°C for 15–60 minutes Assembled DNA FIGURE 1:The typical workflow using the NEBuilder HiFi kit showing various input DNA types, required overlaps, and general reaction conditions.

J Vis Exp. 2021 Feb 24;(168). doi: 10.3791/61937.ABSTRACTThe relative positioning of cells is a key feature of the microenvironment that organizes cell-cell interactions. To study the interactions between cells of the same or different type, micropatterning techniques have proved useful. DNA Programmed Assembly of Cells (DPAC) is a micropatterning technique that targets the adhesion of cells ...

Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids ... This work soundly demonstrates the capability of fabricating modular cascaded circuitry for driving SNA assembly in sophisticated systems, and modulating or programming the molecular machinery for developing an autonomous system.

MIDAS: Modular Idempotent DNA Assembly System: Van Dolleweerd et al., 2018: Allows nesting between existing parts. Allows genes in either orientation. Uses pheS negative marker in Level 2 vectors – unlike ccdB, this does not require special E. coli strain. Assembled 7 …

Here we report a striking effect displayed by "modular primers," which consist of hexamer or pentamer oligonucleotide modules base-stacked to each other upon annealing to a DNA template. Such a combination of modules is found to prime DNA sequencing …

S1 Designer structures assembled from modular DNA superbricks Xue Lia#, Donglei Yanga#, Bo Koub#, Luyao Shena, Haofei Lia, Pengfei Wanga* a Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127,

Request PDF | Monochromatic Fluorescent Barcodes Hierarchically Assembled from Modular DNA Origami Nanorods | With the rapid advancement of fluorescence …

The human immune system consists of a large number of T cells capable of recognizing and responding to antigens derived from various sources. The development of peptide-major histocompatibility (p/MHC) tetrameric complexes has enabled the direct detection of these antigen-specific T cells. With the goal of increasing throughput and multiplexing of T cell detection, protein microarrays spotted ...

A self-assembled, modular DNA delivery system mediated by silica nanoparticles. Luo D (1), Han E, Belcheva N, Saltzman WM. Author information: (1)School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA. Due to the growing concerns over the toxicity and immunogenicity of viral DNA delivery systems, DNA delivery ...

Request PDF | Designer Structures Assembled from Modular DNA Superbricks | Self-assembled DNA structures consisting hold great application potentials in many fields. DNA structure of a specific ...

Monochromatic Fluorescent Barcodes Hierarchically Assembled from Modular DNA Origami Nanorods ACS Nano ( IF 15.881) Pub Date :, DOI: 10.1021/acsnac03796 Victor Pan, Wen Wang, Ian Heaven, Tanxi Bai, Yongxin Cheng, Chunlai Chen, Yonggang Ke, Bryan Wei

versatile modular DNA assembly toolkit for E. coli synthetic biology based on Golden Gate cloning. Here we introduce BacilloFlex, an extension of the EcoFlex assembly standard to B. subtilis. Transcription units flanked by sequences homologous to loci in the B. subtilis genome were rapidly assembled by the EcoFlex standard and

assembly of biological components. The outcome of introducing a rationally designed synthetic DNA construct into a cell is difficult to predict. This creates the need to test multiple permutations to obtain the desired outcome. A greater emphasis on the modular design of DNA parts enables the assembly

DNA Aptamers for Rapid COVID-19 Testing: Interview with Steven Goh, CEO of Achiko and Dr. Michael Edel, Inventor of AptameX. Iron Oxide Nanoparticles Suppress Oral Biofilms.

Icosahedral DNA Nanocapsules by Modular Assembly † Dhiraj Bhatia, National Centre for Biological Sciences, TIFR, GKVK, Bellary Road, …

DNA-encoded chemical libraries (DELs) are increasingly being used for the discovery of protein ligands and can be constructed displaying either one or two molecules at the extremities of the two complementary DNA strands. Here, we describe that DELs, featuring the simultaneous display of two molecules, can b

A solution to this problem is provided by standardizing the parts and the assembly strategy, combined with the use of Golden Gate cloning for DNA assembly at each cloning step. This strategy is employed by the modular cloning system MoClo (Engler et al., 2014 ; Weber, Engler, Gruetzner, Werner, & Marillonnet, 2011 ; Werner, Engler, Weber ...

protein or promoter fusion, and subsequently the functional DNA modules can be assembled into the linear recipient backbones by HindIII cut-ligation (Figure 1a). FIGURE 1 Overview of the modular plasmid assembly system. (a) Schematic diagram of the assembly of the modular plasmids. Red

Abstract. The bryophyte Marchantia polymorpha, has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications.We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for …

A modular and hierarchical DNA assembly platform for synthetic biology based on Golden Gate (Type IIS restriction enzyme) cloning is described. This enabling technology, termed MIDAS (for Modular Idempotent DNA Assembly System), can be used to precisely assemble multiple DNA fragments in …

A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types. Frontiers in Microbiology, 2015. Mark Lever. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper.